五步蛇 meaning in English
[动物学] long-noded pit viper; ancistrodon acutus
Examples
- The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids , which included a signal peptide of 18 amino acids , a proposed propeptide of 171 amino acids , a partial mature protein of 74 amino acids . the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan , but it only contains partial amino acids of mature protein . the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province
结论： 600bp产物和已报道的皖南五步蛇纤溶酶金属蛋白酶氨基酸序列相比较，有90 . 6 %的同源性； 800bp产物中信号肽及前肽和已报道的皖南五步蛇金属蛋白酶很相似，同源性为97 . 9 % ，但仅含有部分金属蛋白酶成熟肽的氨基酸序列，推测此片段为广< wp = 6 >西五步蛇金属蛋白酶原基因的一部分。 - Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province . methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province . different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers
方法：从桂北五步蛇毒腺中抽提总rna ，利用不同的引物，采用一步法（ rt - pcr和pcr在同一管内进行）扩增出不同的dna条带，利用平端连接的方法将pcr扩增产物克隆至pgem - teasy载体，转化大肠杆菌jm109 ，挑选白色菌落提取质粒，用pcr对其进行鉴定，直接利用纯化pcr产物或提取阳性菌落质粒进行测序。