tween-20 meaning in English
聚氧乙烯山梨糖醇酐单月桂酸酯
吐温20
吐温－２０
Examples
- 3 flexible liposome constituted with pc + dona were exhibited under transmission electron microscopy . 4 pfg - nmr analysis indicated that the use of 25 : 1 of tween - 20 : ( - ) c , most of it ( > 90 % ) has been incorporated in the tween - 20 micelle structures
4 、以pnnmr分析了胶束体帅）结构对卜几类物质的荷载量，发现当t een一20使用量为儿茶素卜）的50倍时，绝大部分卜） c （ 90以上）己嵌禾在tween 20形成的胶束体（ 。 - At first , 1 . 67 u g per well mcab all was coated on three wells of a plate , and then 1 . 5 x 1011 phage virion was diluted and added , after incubating with the target , wash away unbound phage by tbst ( 0 . 1 % tween - 20 ) , the bound phage was eluted with ph 2 . 2 tris - gly buffer and amplified , the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles , the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all , collecting and titering the washing phage of last time and output phage in each round , the selective ratio and the false positive rate of each round were worked out , the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning , 11 phage clones were selected after competitive - ellsa , the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced , a clear consensus binding sequence emerged
在本实验中，利用随机12肽库对抗猪瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的单抗a11进行表位筛选，经过四轮筛选以后，随机挑取11个克隆作竞争- elisa检测，结果表明，所挑11个克隆中，有9个克隆能对me2蛋白和a11反应产生抑制作用，抑制率最高可达64 ； dna测序以后经过dnastar软件分析，发现它们的核心序列为anwralsl ，该核心序列与猪瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夹心- elisa检测和western - blotting试验均证明所挑阳性克隆能被a11所识别；人工合成含核心序列的多肽经间接elisa试验证实，也能被a11识别。