tricine meaning in English
两性离子缓冲剂
Examples
- Gene had been successfully transformed and tris - tricine - page revealed that
，将表达载体转化到酵母saccharomyces cerevisiae中， southern杂交证明 - The peptides were extracted , and analyzed using an improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis ( tricine - sds - page ) method
方法通过针刺感染大肠杆菌诱导家蝇各虫态产生抗菌肽，再经研磨、离心等步骤提取这些抗菌肽，用改进的聚丙烯酰胺凝胶电泳使其分离。 - Then bacteria were collected by centrifugation and split by ultrasonic method . the purified products were analyzed by tris - tricine sds - page . the immuno - activity of the recombinant proteins were analyzed by western blot
将离心收集到的菌体超声破壁，离心分离，收集上清液，进行离子交换层析和分子筛分离纯化。 - Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis , biological mass spectrometry and bioinformatics . in 2 - de , we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples . in the identification of 10 proteins of serum and 16 proteins of skin , we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms )
研究方法：采用双向电泳技术获取泥鳅创伤前后血清和皮肤的小分子蛋白图谱，利用基质辅助激光解析飞行时间检测质谱分析( matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry , maldi - tof - ms )和电喷雾离子化串连质谱分析( electrosprayionizationms / ms , esi - ms hs )分别获得差异蛋白点的肽指纹图谱( peptidemassoffingerprint , pmf )和部分序列信息，通过互联网上的expasy服务器中和ncbi的相关软件将这些信息和swissprot数据库进行匹配鉴定蛋白质种类。 - Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well
通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。