transglucosidase meaning in English
转葡糖苷酶
转葡萄糖苷酶
转匍糖苷酶
Examples
- Studies of inactivation of a - transglucosidase by chemical modification demonstrated that cys , asp ( glu ) and his residues were indispensable groups of the a - transglucosidase . partial disulfide bonds were the function of this enzyme
因止，认为cys 、 asp （以）和川s残基为q葡萄糖转苦酶的必需基团，部分二硫键对保护酶的催化功能是必需的。 - A . niger m - l which was screened in our laboratory produced a strongly a - transglucosidase . in this paper , studies on the fermentation conditions , purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation . the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis , the suitable main medium was achieved : which contained 4 % a , 2 % b and 1 % g ; the a . niger m - l was inoculated into 100ml medium in flask , shaking in 33 c at 140r / min for four days , with initial ph6 . 5 and 6 % inocula volume ; adding 0 . 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation , the a - transglucosidase activity amounted to 296 . 05u / ml
本研究以黑曲霉m - 1为出发菌株，对其-葡萄糖转苷酶的产酶影响因素、纯化、酶学性质以及必需基团进行系统的研究，结果如下：通过对影响黑曲霉m - 1产-葡萄糖转苷酶的单因素分析，得液态发酵生产-葡萄糖转苷酶的最适产酶条件为： 4 a ， 2 b和1 g ；在33 ，起始ph值为6 . 5 ，转速为140r min ，接种量为6 ，装液量100ml条件下，发酵4 . 0d ，酶活力达296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷对产酶的诱导作用最大。 - The a - transglucosidase was selectively modified by pcmb , me , edc , clac , acetyl acetone and nbs , and changes in the activities of the enzyme have been detected . the reaction of a - transglucosidase with pcmb , me , edc and clac resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration . the acetyl acetone and nbs were found without inhibition effect
酚丙酮、小涅代唬拍酷亚胺刚b引等化学修饰剂对a葡萄糖转苦酶的几种氨基酸残基进行选择修饰，并测定其酶活力变化，结果表明： pcmb 、 me 、 edc 、 ciac能显著抑制酶的活性，活力的下降与修饰剂的浓度有关，乙酚丙酮、 nbs白巾饰对酶的夺制作用不明显。