trans-golgi meaning in English
转运高尔基氏体
Examples
- These results suggest that the cytoplasmic dynein intermediate chain - like protein exists in lily pollen , and it distributes mainly on either cis - and trans - golgi - associated vesciles
以上的结果表明类细胞质力蛋白中间链存在于百合花粉及花粉管中，并且在百合花粉管中顺面和反面高尔基体附近的囊泡膜上均有分布。 - Rab7 mediates transport of proteins from early endosome to late endosome or fusion of early endosome with late endosome , while rab9 mediates the retransport of m6pr from late endosome to trans - golgi network . the gtp / gdp - binding ability and intrinsic gtpase activity of rab proteins make them exist in rab - gtp or rab - gdp status . the switching of rabs between gtp - bound ( active ) and gdp - bound ( inactive ) forms requires other regulatory proteins , including guanosine exchange factor ( gef ) , gdp dissociation inhibitor ( gdi ) and gtpase activating protein ( gap ) , and lays the foundations of rab function
Rab蛋白家族成员和其他小g蛋白一样可以结合gtp gdp ，在自身gtp酶的水解作用和其他蛋白( gef 、 gap 、 gdi )的调节作用下发生gtp (活化状态)和gdp (无活性)结合形式的转变；同时rab蛋白也在膜结合性( membrane - bound )和胞浆可溶性( cytosolic )之间变化，一个变化过程对应着一个功能循环，接着rab蛋白回到初始状态参与下一个循环。 - Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin , diphtheria toxin , shiga toxin and pseudomonas exotoxin ( pe ) . a number of these toxins are transported to trans - golgi network ( tgn ) , and in many cases such transport to the tgn is required for the translocation and cytotoxicity . in deed , 5 % of the ricin endocytosed by cells has been shown to reach the tgn
蓖麻毒素进入细胞的机理不甚明了，一般认为是rtb先与细胞膜受体结合，主要经过受体介导的内吞作用进入吞噬体，然后沿着内体、高尔基体、内质网等逆向分泌途径，有序地运输到内质网，最后从内质网转位进入细胞浆，在胞浆内攻击核糖体，从而抑制蛋白质的合成，导致细胞死亡。 - It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin , suggesting that the intracellular compartment may be an important part of the uptake pathway . ricin enters the cells by receptor - mediated endocytosis , followed by translocation across the membranes of intracellular organelles . in this study , a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction
本实验为重点研究反式高尔基体在毒素细胞内转运过程中的作用，我们设计了一个反式高尔基体的靶向信号肽基因yqrl ( tyr - gln - arg - leu )连接在rta分子的羧基端，用原核表达蛋白体系，纯化并测定rta - yqrl与rta蛋白对hela (人宫颈癌细胞) ， wish (人羊膜上皮细胞) ， skov - 3 (人卵巢癌细胞)三种细胞的毒性作用。 - By sds - page and immuno - blotting , the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen . subsequently by immuno - gold labeling and transmission electron microscopy , we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles . statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
对分离纯化的百合花粉及花粉管中高尔基体组分进行sds -聚丙烯酰胺凝胶电泳和免疫印迹发现，抗鸡脑细胞质力蛋白中间链单克隆抗体在67kda处有较强的免疫交叉反应；进而通过免疫金标结合电子显微镜观察发现，大多数类细胞质力蛋白中间链存在于高尔基体附近的囊泡膜上；统计结果表明，类细胞质力蛋白中间链在顺面和反面高尔基体附近囊泡膜上的分布机率大致相等。