ti质粒 meaning in English

plasmid ti
ti plasmid

Subsequently , the recombined plasmid was transformed to the strain jm109 of escherichia coli . , which was picked up and was ligated to pbi121 . as a result , the plant expression vector of pbi121 - ubic was constructed successfully
提取质粒，连接重组到经相同酶切的ti质粒pbi121上，构建了植物表达载体pbi121 - ubic ，并将其转入根癌农杆菌lba4404中。
The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing . but difference was found at 3 bases of the sequence from the reported in genbank . then , an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404 . transgenic tomato were screened by their ability of growing on media containing kanamycin
本实验采用pcr方法从番茄花cdna文库中克隆到叶绿体shsp基因，经测序证实与genbank中已发表的序列在编码区相差2个碱基，其中一个碱基导致1个氨基酸的改变。将叶绿体shsp基因定向克隆于带有组成性表达启动子camv35s的植物表达载体prok中，冻融法转化农杆菌lba4404 ，利用叶圆盘法对番茄进行ti质粒介导的遗传转化。