t7rna聚合酶 meaning in English
t7 rna polymerase
Examples
- To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根据端粒酶htert基因1573 ? 1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna 。 - Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
结论：以带t7启动子部分双链dna为模板，用t7rna聚合酶体外合成出的shrna产量较高，纯度较好，是一种简便、高效、低成本的短链rna的制备方法，适合于普通实验室用来进行短链rna的合成和rna干扰实验。 - 1 . expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes , an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro . the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
将cry基因的高保守区的cry a ( a ) ecog - f片段插入带有t7rna聚合酶启动子的质粒pselect - 1 ，获得了能在体外转录的rna探针载体pbpl - 1 ，用该载体制备的rna探针具有特异强，背景清楚，省时省力等优点，已成功地用于苏云金芽胞杆菌的分子生物学研究和特异菌株的筛选。 - Using pcr technology , a 2 . 4kb dna fragment , part of tryptopanase operon , containing a promoter , a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e . coli k - 12 , was cloned to pmd18 - t vector . the dna sequence is the same as which was published on science
为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个dna片段克隆到pet28c质粒的bamhi和hind位点上，使该片段受t7rna聚合酶的启动子控制，然后转化噬菌体de3的溶源菌bl21 ( de3 ) 。 - To prove that the cloned dna fragment can express tryptopanase , a new plasmid pet28c - tnaa , in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ) , a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter , which is inducible by iptg
用iptg诱导表达t7rna聚合酶，以表达质粒上的目的基因。在葡萄糖存在的条件下，用常规方法发酵和诱导( 37 1mmiptg ) ，发现表达的蛋白质条带的分子量与理论上计算的分子量一致。但是发酵液中检测不到吲哚，表明虽然表达了目标蛋白，但表达的蛋白质没有酶活性。