supf meaning in English
祖普夫
Examples
- Our previous work identified delayed mutation occurred at target supf trna gene in plasmid transfected into cells pretreated with low concentration of alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng )
在收回的质粒上我们检测到了延迟发生的、高于对照5倍以上的突变。由于w在细胞中的半寿期仅为1 - Direct damage on supf trna gene can be neglected because half - life of mnng is 1 . 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours . therefore the mutagenesis is not lesion directed
20m的mnng经洗涤后的极微量残留经过10 ? 20多个半衰期的降解后，己不足攻击转人的qdna分子，因此这种突变显然是发生在mnng直接攻击部位以外的正常碱基上。 - In our laboratory , a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0 . 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0 . 2m mnng for 2 . 5h , then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture . we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells . this kind of mutation did not occur immediately after mnng exposure
我们实验室曾用一特殊的突变检测系统，直接证明dna损伤剂可在哺乳动物细胞诱发非定标性突变：首先用低浓度( 0 . 2 m )的短寿烷化剂mnng (半寿期为1 . 1hr )处理细胞2 . 5h后，继续培养24h ，将重组有用作突变检测的靶基因supftrna基因的穿梭质粒pz189转入细胞复制，发现在未受致癌物直接攻击的穿梭质粒中有较自发突变率高5倍以上的靶基因突变。