sry基因 meaning in English
sry
Examples
- Part two : studies l . the sox gene of rana rugulosa wiegmann was amplified by using the technique of pcr with a pair of primers which specially amplify the conserved motif ( hmg - box ) of human sry gene
二、研究报告1 、采用pcr技术，以特异扩增人sry基因hmg - box保守区的一对特异引物扩增了虎纹蛙sox基因，优化了扩增条件。 - Part two : studies ; l . the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene . the amplification band was observed in both male and female dinodon refozonatum , whose length was consistent with that of human sry gene , which about 220bp . the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human , and there was a few differences between male and female dinodon refozonatum . 2 . using a pair of degenerate primers based on the conservative region , hmg - box , of human sry gene , six different fragments were amplified from either female or male dinodon refozonatum , then cloned by using pmd18 - t vector and sequenced
结果显示： ( 1 )赤链蛇基因的扩增片段与人sry基因扩增片断大小相同，为220bp左右； ( 2 )雌、雄赤链蛇sox基因的扩增片段大小虽然与人的相同，但其单链迁移率与人的有较大差异，而且雌雄个体间有明显差异，预示该基因的dna序列在雌雄个体中可能有差异； 2 、参照人sry基因hmg - box保守区的序列，又设计一对兼并引物，扩增了赤链蛇的sox基因，并对扩增产物进行了克隆和测序。 - Using a pair of degenerate primers based on the conservative region , hmg - box , of human sry gene , tow different fragments of sox gene , essox3 and essox22 were amplified from female and male eriocheir sinensis , the sequence results indicated that essox3 and essox22 shown high homology to human sox genes , and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 % , respectively . it might be concluded that sox gene was highly conservative in phylogenesis
二、研究论文1 、参照人sry基因hmg - box保守区的序列，设计一对兼并引物， pcr扩增了中华绒螯蟹的sox基因,并对扩增产物进行了克隆和测序。结果在雌雄个体中筛选出两个不同的sox基因essox3和essox22 ，其dna序列和编码的氨基酸序列与人相应sox基因的相似性分别为84 % 、 85 %和97 % 、 81 % ，显示该基因在进化上具高度的保守性。 - Using the hprt gene as a positive control , our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist . female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too . while since the 4 - cells period , xist is constantly transcribed until blastocyst stages
然后利用实验一确定的pcr条件，以hprt为阳性对照，用巢式rt - pcr对小鼠早期胚胎进行xist基因的转录分析，结果发现，转录sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录xist基因；不转录sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2 -细胞期的过程中， xist基因一直不转录，但是，从4 -细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录xist基因。