sequence-specific meaning in English
序列特异的
Examples
- The back - exchange process is actually complex and deserving of analysis in a sequence - specific manner
返交换实际上很复杂，需要进行序列特异性的分析。 - The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究设计以c tamrna为模板的反义cdna片段，从c ta基因5 ’端第94位到500位核苷酸段设计引物，目的片段407bp ，覆盖第116和188位两个aug密码子，也包含了第一外显子和第二外显子间的剪接位点：用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ；腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒，用氯化铯密度梯度离心法获得纯化的高滴度腺病毒；进行体外基因转移，分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞，观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用，从而达到调控mhc -类分子表达的目的。 - In this paper , a series of transition metal complexes were synthesized ; interaction and reaction mechanism between complexes and dna were studied . in addition , we synthesized and analyzed a sequence - specific cleavage reagent , in which oligodeoxynucleotide was recognizing group . detailed work mentioned below were carried out : 1
本文在查阅大量文献的基础上，以1 ， 10 -邻菲咯啉和1 , 10 -邻菲咯啉- 5 , 6二酮为配体合成了一系列过渡金属配合物，详细研究了它们与pbr322dna的相互作用及其机理。