saci meaning in English
非洲工商业公司
萨西
Examples
- On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7
设计具有ndei和saci单限制性酶切位点的vp7基因引物，通过pcr扩增出vp7基因并经测序验证。 - And wibdv specific rt - pcr also uesed to detected these samples in the years from 1993 to 2000 , there are 8 strains belong to wibdv and 13 belong cibdv . two strains ca n ' t typing for they can be cleaved with both of enzymes sspl and saci .
结果发现1993年至2003年的病料或分离的毒株中，有8株确定为vvibdv ， 13株踊定为cibdv ，另外，有两株不能确定。 - Dna band is about 600 bp when p1asmid was digested by sacii and saci enzyme , and is equal to that of hbsag gene ; but it is about 700bp when plasmid was digested by xhoi and saci enzyme , and is equa1 to the weight of s , . / hbsag fusion gene
经sacll和saclk切电泳可见约600hp的dna带，与hbsag基因大小相当；用xhol和sacl切电泳可见约700hp的dna带，同s …与hbsag基因融合片段的大小相等说明s ； 。 - But the vvidbv strain gx8 / 99 that had been identified by gui only have saci side , and no sspl , the reason need farther research . according to the specific different sequence between cibdv and vvibdv in vvp2 gene . we also designed two sets of primers ( cibda + cibds and vvibda and vvibds )
另外，针对不同强弱毒株的1 v的pz高变区序列的差异：设计合成了vvibdv的型特异性引物（ ibda vvibds人建立型特异的rt pcr ，只需一次反应即可区分cibdv和vvibdv 。 - Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control
选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p