rprv meaning in English
遥控研究机
Examples
- Minimum dose of protection of recombinant pseudorabies virus rprv - gp5 against pseudorabies in sheep
对伪狂犬病最小免疫剂量的测定 - To differentiate the animals immunized with rprv - ha and those naturally infected with prv or sfv in future , two differentiating diagnostic methods were developed based on recombinant viral antigens
为了区分此重组病毒疫苗免疫的动物和自然感染prv和siv的动物，本项研究利用基因工程抗原建立了两种分子鉴别诊断方法。 - After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞，采用甲基纤维素固定病变， x - gal染色，经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株，命名为rprv - lacz 。 - The expression of ha in vero cells infected with rprv - ha was detected by western - blot . the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha . the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza
结果表明所获得的重组病毒（ rprvha ）遗传性状稳定，在培养细胞中能稳定的表达与sivha具有相似生物学活性的外源蛋白，为进一步制备抗猪流感的重组伪狂犬病毒活载体疫苗奠定了基础。