rescreening meaning in English
重新筛分
Examples
- In this paper , a strain was selected with high esterase activity . its characteristics of production and enzymology and separation of l - lactic acid were investigated . through primary screening and rescreening , a fungus , f - 16 , with high esterase activity was selected
本文选育出了高活性酯酶产生菌株并对该菌体的产酶性质、酶学特性及酶对乳酸乙酯的手性拆分后产物分离作了一定的研究。 - The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium . after being isolated and purified , these strains were inoculated into fermented medium , shaking in 28 c at 220r / min for 5 days , then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2 . 5 . the result showed there were 24 strains with higher enzymatic activities among the 102 strains , after the rescreening , 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase , of which , enzymatic activity of the strain 14 was the highest , reaching 53 . 86u / ml , and it was preliminarilly identified as aspergillus . niger , then numbered as aspergillus . niger 14
用植酸钙选择性平板培养基从土样中筛选出了102株能产透明圈的菌株，经分离纯化后，接入液体发酵培养基， 28 、 220r min发酵5天，在37 、 ph2 . 5条件下用钒钼酸铵法测定其所产植酸酶的活力，结果显示，酶活较高的有24株，经再次摇瓶复筛后，酶活大于15u ml且产酶性能稳定的共有7株，其中以14 ~ #菌株的酶活最高，可达53 . 86u ml ，经初步鉴定为黑曲霉，编号为aspergillus . niger14 ~ # 。