renatured meaning in English
复性的
复原的
Examples
- When the denatured reduced egg white lysozymes were renatured by dilution method , an observable amount of aggregate precipitate could be immediately formed
当用复性液稀释复性还原服变性蛋白溶菌酶时，会迅速产生可观量的沉淀。 - We also synthesized w - ctx mviia and studied its renaturation under air oxidizing and gsh - gssg condition . we researched the physiological activities of the renatured peptides on isolated mouse phrenic nerve - diaphragm preparations or on isolated mouse spermaduct preparations
另外还合成了- ctxmv a ，并用空气氧化法和谷胱甘肽法，研究了多种条件下的复性情况。 - Ii ) the renaturation of synthetic hpab was carried out in glutathione oxidation / reduction solution ( l : 10 ) . the renatured synthetic hpab could kill escherichia coli , pseudomonas aeruginosa and staphylococcus aureus on lb agar plates . thirdly , construction of artificial mutants of hpab
3 .突变体的设计及其重组杆状病毒构建:采用同源模建法分析hpab一p的空间结构，并以此结构为基础，设计hpab一p突变体hpab一p38和hpab一p34 。 - Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody . our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4 . after purified and renatured , r hpf4 prepared by our methods has bioactivity like wide hpf4 . our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells
我们构建的rhpn原核高效表达系统经m page及凝胶密度扫描分析结果表明， rhpf4表达量占菌体总蛋白量的25 30 ，较原表达克隆pt7 7 rhpf4提高了近80倍，经快速高效的包涵体分高纯化工艺和复性工艺， rhpf4具有野生蛋白活性。 - The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that , by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates , and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant
沉淀和上清液的不连续十二烷基硫酸钠聚丙烯酰胺凝胶电泳( sds - page )和高效凝胶排阻层析分析结果表明，还原脲变性蛋白溶菌酶在稀释复性过程中除了能够复性成天然态蛋白溶菌酶分子外，还会形成可溶的蛋白溶菌酶分子二聚体和三聚体，二聚体和三聚体主要是靠分子间二硫键的错配连接而成的；可溶的蛋白溶菌酶分子二聚体之间通过非共价键相互作用而形成集聚体沉淀，而可溶的三聚体溶菌酶分子则仍处于复性液上清液中。