recombinase meaning in English

重组酶

In the present study , we develop an efficient method to be used to examine recombination efficiency of transgenic plantlet . two constructs , one containing cre recombinase under the control of
对cre在转基因个体中介导的重组效率进行了研究。构建了含有
To abolish transcription efficiency affected by copy number or integration site in chromosome , all the vectors were integrated into frt site by co - transfecting with an flp recombinase producing plasmid
应用w七stemblot对其中的20个克隆进行检测，选出一个caspase一3表达量最低的细胞克隆a6 。
After the examination of pcr , one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product , a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut
经pcr检测后，有一个不发光细胞克隆扩增出了与对照质粒ploxifn相同的约1000bp的条带，表明发生了替换反应；另一个不发光细胞克隆认为是在cre重组酶的作用下其内部自行发生了剪切作用，剪切了gfp基因，从而只扩增出了载体序列和一个lox位点的约500bp的条带。