ppsa meaning in English
泛太平洋外科协会
清管产品和服务协会
Examples
- Ppsa and tkta are the key genes in central metabolism of aromatic amino acids biosynthesis . ppsa encoding phosphoenolpyrucate synthetase a ( ppsa ) which catalyzes pyruvate into pep ; tkta encoding transketolase a which plays a major role in erythrose - 4 - phosphate ( e4p ) production of pentose pathway
Ppsa基因编码磷酸烯醇式丙酮酸合成酶a ( ppsa ) ，该酶催化丙酮酸合成磷酸烯醇式丙酮酸； tkta基因编码转酮酶a ，该酶在磷酸戊糖途径中生成4 -磷酸赤藓糖起主要作用。 - When both genes were co - expressed in e . coli , the activity of ppsa varied from 2 . 1 - 9 . 1 fold comparing to control , but the activity of tkta was relatively stable ( 3 . 9 - 4 . 5 fold ) . whatever the two genes were expressed respectively or cooperatively , both could promote the production of dahp , the first intermediate of the common aromatic pathway , but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective . the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp , and what ' s more , when multigenes co - expressed , the recombinant which has coordinated enzymes activity is optimum
莽草酸途径的最优化和整体调控基因csra的敲除正是上述改变的分子基础，同时也为三种芳香族氨基酸的基因工程菌的构建打下了基础; 7 .在国内外首次实现了共同途径限制性底物关键酶ppsa刁无‘及arog与分支途径关键酶基因phea的串联高效表达，所构建的重组质粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分别比对照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比较协调一致; 8 .将ptga导入到筛选的基因敲除和基因替换菌株大肠杆菌31884 c甲b中，摇瓶发酵证实比以往所构建的基因工程菌株具有较高的phe产量和糖转化率率，分别为0 . 448 %和22 . 4 % 。 - We amplified ppsa and tkta from e . coli k - 12 by pcr and constructed recombinant plasmids of them in pbv220 vector containing prpl promoter . because of each gene carrying pl promoter , four productions of ligation were obtained , that are ppt - , ppt - , ptp - , and ptp - . the results of sds - page demonstrated that the bands at 84kd and 73kd were more intensive than the same ones of the controls
3 .从大肠杆菌中克隆了莽草酸途径关键酶基因aro刁、 aroc 、 arob及整体代谢调控基因csra及其侧翼序列;从质粒pet28a中克隆了kan抗性基因; 4 .构建了基因盒子aroaar口carobkan ，在其前面加入了强启动子ptac ，初步证明了pta 。