poros meaning in English
波罗什
波罗斯
Examples
- To reveal the reason of above phenomenon , we purified the eluant by poros 20hq ion - exchange chromatography and got five proteins . one of them ( hq - s ) was proved to can be proteolytic
为弄清这一现象的产生原因,将嗜硫色谱洗脱峰通过poros20hq离子交换色谱进一步分离,获得5个分子量非常接近的组分。 - 3 . 2 . 1 . 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25 , and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography . the other was a p - glucosidase ( ec . 3 . 2 . 1 . 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography . the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433 . 38 hj / mg
-葡萄糖苷酶对水杨素的比活力为597 . 12iu mg ,并对其专一,不能水解棉花和羧甲基纤维素钠;分子量为117 . 5kda ,加dtt后分子量不变;该组分最适ph和温度分别为4 . 5和70 ,在ph5 . 0 、 50下对水杨素钠的米氏常数km为3 . 73mg ml ,最大反应速度vm为0 . 088mg葡萄糖( ml ? min ) ;与文献中从黑曲霉中分离的-葡萄糖苷酶比较后发现,该组分是一个新的-葡萄糖苷酶。