pgem meaning in English
前列腺素e代谢产物
Examples
- Then the pcr product was purified , ligated into pgem - t vector by ta cloning
筛选阳性克隆，测序，大量制备序列完全正确的质粒。 - The pcr product was inserted in e . coli clone vector pgem t - easy , and sequenced . a new subtype poifn - a was obtained
Pcr产物经纯化回收后，插入pgemt - easyvector ，经序列测定证实克隆了一新亚型猪干扰素。 - Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 - Search the snps in wnk4 genomic sequences design the primers to get the unique pcr products of wnk4 gene which cover the full length genomic sequences
Dna的pgem一t载体为模板，用含有ha标签及酶切位点的上下游引物进行pcr扩增，酶切pedna3 - Put this fusion gene under the control of the promoter of ie1 gene , and then an eucaryotic cell expression plasmid vector pgem / ie1 " - gfp - actin resulted
将该融合基因置于ie1基因启动子的控制之下，构建成了真核表达质粒pgem ie1 - gfp - actin 。