pbv meaning in English
对虾杆状病毒
经皮气囊瓣膜成形术
Examples
- When the jm109 with pbv / hpk - 5 cultured at a rotating shaker at 30 ? for 3 hours , ph 7 . 2 , 100ml medium in 500ml flasks , induced at 42 ? for 6 hours , the recombinant protein of rhpk - 5 showed good stability generating
山西医科大学2002届硕士学位论文3 .采用“超声+裂解缓冲液”的方法破碎菌体， 3 %脱氧胆酸钠有助于溶解少量不溶性的杂蛋自。 - Four of the six resulting recombinants contained two genes arog and qutb , the other contained three genes arog , qutb and arob . the catalytic activities of both ds and qdhase increased diffierently , but enzyme activities of the recombinant harboring pbv - prpl - qutb - pl - arog were best
六种重组子都实现了目的蛋白的表达，表达产物ds和qdhase酶活性有不同程度的提高，其中pbv - prpl - qutb - pl - arog串联表达重组子表达产物二种酶活性ds和odhase都比较高，是优化的重组子。 - Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease . the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning . through sds - page , bioactivity and function analysis of expressed protein , the function of phaa , phab and phac was confirmed
菌株的亚克隆基因组片段中，分离出phaa 、 phab和phac三个基因片段，定向克隆至原核表达载体pbv220上，构建了三个原核生物表达载体pbv - a 、 pbv - b和pbv - c ，通过对表达载体诱导表达，表达蛋白产物的sds - page分析、生物活性与功能分析，确定了基因phaa 、 phab和phac的生物学功能。 - Phaa , phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease . the expression vectors of pbv - a , pbv - b and pbv - c were constructed by orientaional cloning . indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs
将phaa 、 phab和phac片段双酶切后，定向克隆至原核表达载体pbv220 ，构建了三个原核表达载体pbv - a 、 pbv - b和pbv - c ；经酶切分析表明，所克隆的三个基因phaa 、 phab和phac置于表达载体的正确阅读框架下。 - Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5 , rhpk - 5 ) protein was recognized by mab same as native hpk - 5 . the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5 . section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system , the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5
Coli )作为宿主，经sds - page分析，筛选表达量最高的菌株作为发酵用工程菌株；用western - blot方法鉴定hpk - 5因子的免疫学活性；用摇瓶发酵的方法，研究发酵培养基的体积(溶解氧) 、组成成份及诱导起始时间和诱导持续时间对目的蛋白表达量的影响，优化hpk - 5基因工程菌的表达条件。