orit meaning in English
奥里特
美洲区域工人组织
瑞亚太
Examples
- A 1 . 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e . coli / streptomyces shuttle vector with conjugation function ( containing orit gene ) . as a result of above procedures , a recombinant plasmid pid03 was obtained
将1 . 5kb的安普霉素抗性基因片段插入到aved基因中的nrui酶切位点，再将此灭活的aved基因片段插入到具有接合转移功能(含有orit基因)的链霉菌?大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pid03 。 - Based on a 3 . 1kb pst i fragment of genomic dna of a wild s . avermitilis , a 1 . 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3 . 1 kb fragment , then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401 . competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因组dnapsti片段为基础，将1 . 5kb的安普霉素抗性基因片段插入到avec基因中的sphi酶切位点，再将此插入失活的avec基因片段连接到具有接合转移功能(含有orit基因)的链霉菌-大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pc05 。 - A fragment , containing 2 . 0kb cloned 5 . tenebrarius dna and reported genes of erme and xyle , was inserted in plasmid phz132 ( an e . coli - streptomyces shuttle plasmid incorporating orit from rk2 ) to construct disruption plasmid pzxb0l4 . the plasmid was transformed into e . coli et12567 ( puz8002 ) to construct recombinant e . coli et12867 ( puz8002 , pzxb014 )
将克隆到的链霉菌dna2 . 0kb片段以及报告基因erme 、 xyle插入到具有orit的大肠杆菌?链霉菌穿梭质粒phz132中构建接合转移质粒pzxb014 ，并将其转入大肠杆菌et12567 ( puz8002 )中，获得供体菌et12567 ( puz8002 ， pzxb014 ) 。 - To facilitate the detection of gene replacement , apr gene was placed in the middle of the two inserts . orit from e . coli plasmid rk2 was also incoporated into the vector , therefore , pij903 derivatives can be mobilized from e . coli into streptomyces sp . fr - 008 by either bi - parental conjugation or by conjugation between streptomyces
为了在克隆完整基因簇时避免这两方面的影响并能随机地获得dna大片段，我们将第二代yac载体pjs97 - pjs98改造成为适合于在白林泉链霉菌基因组中dna大片段的基因置换与克隆链霉菌中分析被克隆大片段功能的基因置换载体。