multicopy meaning in English
多拷贝
Examples
- Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide , which was identified by sds - page and western blotting
培养多拷贝插入的酵母细胞，用甲醇诱导目的蛋白的分泌， sds - page和westernblotting鉴定表达蛋白。 - Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies
重组质粒转化巴氏毕赤酵母， g418筛选出多拷贝插入的单克隆，甲醇诱导多拷贝插入的单克隆酵母细胞分泌目的蛋白，培养液上清经sds - page电泳分析，在蛋白质印迹中检测到培养液上清有一表观分子量为36kd ，能与羊抗hcmv多克隆抗体发生发应的条带。 - Considering alcaligenes faecalis pencillin g acylase ( afpga ) , which possesses the attractive characteristics for beta - lactam antibiotics conversions , the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga . both the constructed plasmids psmlfpga and pkkfpga contained the pga gene , trc promoter and rrnb transcript terminator , differ in the replicon and antibotic marker , pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker . while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文将粪产碱杆菌青霉素g酰化酶( afpga )基因构建表达载体pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc启动子、青霉素g酰化酶基因、 rrnb转录终止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷贝复制子；而psmlfpga含有四环素抗性基因和p15a中拷贝复制子。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。