ligase meaning in English
n.
【生物学】联结酶。
Examples
- We have identified in s . cerevisiae distinct ubiquitin - ligase complexes that define different erad pathways
我们在酵母体内发现了一种参与不同erad途径的特定的泛素连接酶复合体。 - Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate
连接酶:可将两个底物结合在一起的酶,此过程需要atp或其他核苷三磷酸供能。 - Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接,并导入dh5感受态细胞中,构建成cdna文库。 - As bases are added by polymerase to the starting point of a new complementary strand , known as a primer , or recognized by ligase as a match , the template ' s sequence is revealed
当聚合酶将一个核苷酸加在新互补链的起始引子之后,或接合酶认定某段核苷酸链与原始模版配对,就可利用这些反应来得出原始模版的序列。 - 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒,用kpni和bamhi酶切,分别回收cpti片断和酶切后的载体片段,用t _ 4连接酶连接构建成中间载体pgem - cp 。