gll meaning in English
大赏典
Examples
- Government land licence gll
政府土地租用牌照 - 9164623033 dear value customer , gll _ prices t are being updated . final prices need to be confirmed by phone or email dthank you for chzoosing america asia
很抱487871歉,各位尊贵的311616顾客们,我们正在更新所有2007年的团费,正确团费以电话? f ? ?电邮确? - ? ?准,不便之处,敬请见谅 - The errors in calculating derivatives for the gll collocation points are evaluated , which can be alleviated from o ( ( n4 ) to o ( ( n2 ) by the double - precision method proposed in the present paper , where ( denotes the machine precision and n the order of the interpolation polynomials in the elements
文中对gll配置点下的求导误差进行了分析，提出的双精度方法可以将求导误差从o ( ( n4 )减小到o ( ( n2 ) ，其中(为机器精度， n为单元内插值多项式阶数。 - Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation . 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies . 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs , prepare a 50 % slurry for batch purification method , pack column with matrix slurry
三、 gst一hbrp重组蛋白的纯化1 .超声破碎细胞，离心，上清和沉淀进行sds一page电泳分析2 .样品处理提取包涵体，变性后，加人用pbs平衡过的以utal肠onesepb抓脱4b ，室温下孵育3 .以utadtionesepharose4b柱纯化以utad云onesepharose4b柱的准备根据毛山lel ，决定纯化所需的gll卫ta1如oneseph娜se4b的柱床体积，用预冷的1xpbs清洗cldtathionese户， se4b ，得到50 %的基质