g-100 meaning in English
葡聚糖凝胶
Examples
- In order to attain bioactive glycoprotein , glycoprotein of the leaves of camellia sinensis , was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography , by cona - sepharose 4b affinity chromatography
为了纯化天然糖蛋白，进行了茶树叶糖蛋白的cona - sepharose4b亲和层析，从sephadexg - 100凝胶过滤收集的粗糖蛋白中，分离茶树叶糖蛋白。 - 3 . the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100 . the specific activity of the purified enzyme was increased by 37 . 9 folds over crude extract , and the recovery yield was 8 . 9 %
以粗酶液为起始，经硫酸铵分级沉淀、 sephadexg - 100凝胶过滤后，分离纯化了该酶，纯化倍数约为37 . 9 ，酶活力回收率8 . 9 。 - The crude cellulases from liquid fermentation of b - 6 and ass . 3711 were isolated and purified by ( nh4 ) so4 precipitation , sephadex g - 100 and deae - sepharose cl 6b column chromatography . the cmcase components were purified and some of their physical and chemical properties were studied
本文将液体发酵的酶液经硫酸铵分级沉淀、柱层析后得电泳纯cmcase组分，并对as3 . 3711和b - 6来源的cmcase酶解动力学和理化性质作了比较研究。 - The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100 . the sod activity for purified enzyme could reach 4966 iu / mg proteins , and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis . when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0 . 2mmol / l kcn or 0 . 5 mmol / l h2o2 , the active band was still showed at the same position , which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通过sds一聚丙烯酸胺凝胶电泳可知该sod酶的分子量约为20kda .在非变性聚丙烯酞胺凝胶( pagb )电泳后，在凝胶son显色反应液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝胶sod显色反应后在原来的sod酶部位仍然含有sod活性带，这表明利用kcn和h20 :处理并不能抑制500的活性，该sod属于mn一sod 。