fragement meaning in English
碎片塞
Examples
- 4 ) fragement recovery of pcr products pcr products were separated on a 1 . 5 % agarose gel . the expected fragment was recovered by using pcr fragment recovery kit according to the manufacturers instructions
4 、凝胶回收应用pcr目的基因片段回收试剂盒对扩增阳性pcr产物进行凝胶回收，纯化目的dna片段。 - By rt - pcr method . we first selected a pair of primers named 2bp and 4bm which was designed in highly conserved 922 nucleotide region in open reading fram ( orf ) ib from coronavirus . this primer could amplify 11 kinds of coronaviruses . after synthesizing this pair of primers , we amplified the target fragement of 251bp from the panda ' s liver - tissue materials and other different passages of culture of this virus . however , the control cell showed negative result
第一对引物为外层引物，可扩增出1086bp的核酸片段；第二对引物为内层引物，可扩增出515bp的核酸片段。该引物以k378为参考株，含有多个兼并碱基，可扩增出包括k378 、 insavc - 1 、 ccv1 - 71 、 nvsl吉林农业大学硕士学位论文大熊猫犬冠状病毒的分离鉴定及s墓因部分序列的测定和分析等多种犬冠状病毒。 - To construct eukaryotic expression vector of mbl gene with codon 54 point mutation , the target sequence in pgem - mbld plasmid , which conains mbl cdna with codon 54 mutant allele , was amplified by pcr . after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i , the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector , and the recombinant vector , named pci - mbl54 , was obtained . the pci - mbl54 , digested with restriction enzymes , was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本实验以ggc54gacmbl突变为研究对象，选用真核表达质粒pci - neo ，根据已构建好的含54位密码子突变型mbl基因t载体的结构，设计合成新的引物， pcr扩增54位密码突变型mbl基因，凝胶回收，双酶切pcr产物和pci - neo质粒， t4连接酶连接，将前者克隆至后者的sma和sal位点，转化大肠杆菌xl - 1blue ，氨苄选择培养。 - In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not
合成该套式引物，选择大熊猫原代病料和病毒各代细胞培养物，经套式( nested ) rt一pcr扩增，可得到一与设计值5巧bp相符的dna片段，经bst一xl ( 590 ， 1110 )酶切鉴定，证明该扩增片断为特异性片段;回收大熊猫肝组织原代病料和细胞培养物第2 、 3 、 29代的ccvfz一ccvrz扩增片段，纯化，送生物公司测序。