dntp meaning in English
三磷酸脱氧核苷
Examples
- 2 . pcr products can be purified by ctab method , which removing the dntp and primers . the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency
2 、采用ctab进行pcr产物的纯化，以去除pcr产物中的引物和dntp ，所得产物可用于与t载体连接反应，以提高连接效率。 - The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents , including total rna , anchor primer , arbitrary primer , cdna and dntp
通过调整ddrt - pcr中总rna 、锚定引物、随机引物、 cdna和dntp等关键试剂的用量，优化了适用于银染检测的ddrt - pcr方法。 - The routine method for inducing protein function evolution is imitating and speeding protein gene mutation . in this research - amylase gene was mutated in vitro by pcr with dntp in which deoxythymidine triphosphate ( dttp ) was partially replaced by 5 - bromo - 2 ' - deoxyuridine - 5 ' - triphosphate ( 5 - bdu ) . the higher the concentration is , the stronger its inducing ability is in a certain range
本研究采用5 -溴脱氧尿苷三磷酸( 5 - bdu )部分取代脱氧胸苷三磷酸( dttp ) ，在pcr的过程中对克隆的野油菜黄单胞菌的-淀粉酶基因进行了体外诱变，诱变结果表明： 5 - bdu浓度越高，诱变越强；浓度越低，诱变越弱。 - 2 . 5 ul of 10 x reaction buffer , 1 . 5 ul 25mm mgcl2 , 0 . 3 ul lomm dntp , 0 . 5 ul taqdna polymerase ( 5 u / ul ) , and lul ( = 20ng ) of primer were used in per reaction . each reaction was overlaid with one drop of paraffin oil . the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec , annealed at 37 1 min , extended at iv b 72 for 2 min and repeated the cycle 45 times , at last , extended at 72 ' c for lomin
等( 1995 )利用rapd标记区分美国东部一杂交地带的蟋蟀的两个姐妹种， allonemobiusfasciatus和a . socius ，并于1998年使用rapd和异型酶标ic做出了这两种蟋蟀的基因连锁图；国内田英芳、郑哲民（ 20of ）首次将rapd技术运用于蟋蟀总科的分子系统学研究中，采用2种引物对7种蟋蟀进行了基困组dna多态性研究，并应用upg问a法构建树状图，椎测系统发生关系