dna连接 meaning in English
dna ligase
Examples
- According to the mutliclone site of pcdnas plasmid , we have used xho i to cut pcdnas plasmid and make its linearized
Pcdna3和doc一ir的酶切片段25经毛dna连接酶作用形成重组质粒。 - Ddp reduced telomerase activity in the cells in a concentration - dependent manner as measured by using the telomere repeat amplification protocol ( trap ) assay
在晕丸癌的研究中发现： ddp的抑制作用是由于此药物进人细胞后和dna连接，形成g pt g的连接方式。 - A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接；用连接产物转化大肠杆菌jml09感受态细胞，置含amp 、 x - gal和iptg的lb平板上培养12 20小时；挑取白色菌落于选择性培养基扩大培养，碱裂解法小量提取质粒dna ，并进行酶切分析鉴定，结果获得整合有prvge基因的重组质粒pugedna ，并与其它prv分离株进行ge基因序列同源性分析。