dna结合域 meaning in English
dna binding domain
Examples
- It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation . a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter
( 465 1079氨基酸段)对sv40启动子明显的抑制活性,因为它只包含dna结合域和核结合位点,说明发挥转录抑制功能的关键是ie180的dna结合域。 - The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中,得到的重组质粒命名为pgbkt7 - g , ?半乳糖苷酶活性鉴定表明g不具有自激活特性,将其转化到酵母菌pj69 - 4a中,再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。