dispase meaning in English
分散酶,中性蛋白酶
中性蛋白酶阀于分散组织培养中的动物细胞
Examples
- If the skin has been put into 4 percent glutaraldehyde for three minutes or more . we are no longer able to get rid of the cells in it . the a - gal in porcine skin as disapered in acellular matrix treated by trypsin , dispase and sds
实验发现4戊二醛对基质交联固定后,若交联时间短,则与不交联组无差异,时间稍长,则胰酶无法脱除细胞。 - Trypsin , dispase or hypertonic saline is used in decelliztion . triton x - 100 and sodiumdodecylsulphate ( sds ) are effective detergent in removing cell debris , but it is unknom that which of these is more effective
在去除细胞碎片方面,文献中介绍以用曲拉通( tritonx 100 )和十二烷基磺酸钠( sodiumdodecylsulphate , sds )为多,对于这两种洗涤剂的选择也有不同看法。 - We treat the porcine skin by 0 . 25 percent trypsin , 0 . 125 % trypsin , 2 . 5 u / ml dispase , hypertonic saline or hypertonic saline - trypsin / dispase . we find that after the skin has been incubated in 0 . 125 percent trypsin for 24h at 4 ? , the cells in the skin are all disintegrated . there are no significant differentiation between the acellular matrix treated by 0 . 125 , 0 . 25 perlent trypsin , 2 . 5 u / ml dispase and hypertonic saline - trypsin / dispase . but the cell ca n ' t be removed by using the hypertonic saline - sds
本研究通过对0 25胰酶不同脱细胞时间处理、不同浓度胰酶处理、 dispase脱细胞法、 im 、 zm高渗盐水脱细胞法、高渗盐水和胰酶或dispase混合脱细胞法的比较确认采用0 12盼胰酶, 4 , 244 。 - In addition , we designed a test : the midgut was removed from spodoptera exigua larvae and was cut longitudinally into halves , the midgut epithelia were cleaned and digested with dispase ii , the pelleted cells in grace medium with 7 % fbs supplemented with 100units / ml penicillin , 100ug / ml streptomycin were cooled and then suspended in r18 - labelled virus
实验结果表明,在幼虫的病毒接种物中添加合适浓度的荧光增白剂( 0 . 01g . ml一’ )不会改变病毒在幼虫中肠组织中的感染部位,却可以提高幼虫对病毒的敏感性,增加了病毒毒力。 - The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell , collegenase i ( 150iu ml - 1 " ) and trypsin ( 0 . 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue , which is appropriate to obtain qinchuan - scalper skin cells
组织块法、分散单细胞及dispase冷消化法单细胞均能获得好的秦川牛皮肤组织原代培养物。其中150iu ? ml ~ ( - 1 )胶原酶i与0 . 05胰蛋白酶( 1 : 1 )同时应用能更好的分离秦川牛皮肤组织,是获得秦川牛皮肤细胞的适宜方法。