digoxigenin meaning in English
地谷新配基
毛地黄毒苷
洋地黄毒苷，地高辛配基
异羟基洋地黄毒苷
异羟基洋地黄毒甙元
异羟洋地黄毒苷配基
异羟洋地黄毒苷元
Examples
- Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome
2 。对点杂交为阳性的植株进行southernblot分析，转化植株检测到了杂交带，除预期的1 - The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank . concentration of tp was analyzed after amplification invitro by rt - pcr , purified by low melt agarose and labeled by digoxigenin
根据genbankth - 98株序列设计第2对引物，应用rt - pcr方法体外扩增该片段(命名为tp ) ，琼脂糖凝胶纯化后测定其浓度和纯度，进行非放射性地高辛标记。 - The expressed product was lysised with supersonic wave and purified by sds - page . elisa analysis revealed that the antigenicity of the vpi protein has been detected . the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ) . then the probe hybrid with 5d , 10d , and 20d postinfection brain tissue of chicken . the results of the ish showed that the positive signal was found in 3 cases , while control group was negative . there has been a reasonable correlatien between this method and other detection test
经超声波裂解，用尿素溶解包涵体，电泳纯化后，利用elisa检测vp _ 1外壳蛋白，表明具有一定抗原性；第四部分：应用原位杂交检测aevi用dig标记的探针与sd 、 10d 、 20d的攻毒鸡脑组织杂交，来检测那v的rna 。 - One was cloning , sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ) , the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
为了鉴别诊断tgev与prcv及对tgev进行流行病学调查，本研究采用原核表达系统( gst融合表达系统)表达tgev纤突蛋白( s蛋白)中含有b和c抗原位点的多肽，并且制备了非放射性地高辛标记的核酸探针，通过斑点杂交( dot - blot )检测tgev核酸rna 。 - 4 . we use half quantification and digoxigenin detection to investigate the expression pattern of ent - kaurene oxidase . we found that it was regulated by development , it expressed high in the fast growing tissue , expanded leaves under a fast growing circumstance also express high level of ent - kaurene oxidase
利用半定量rt - pcr的方法结合地高辛标记法，贝壳杉烯氧化酶在果树中的表达是受发育调控的，其在旺盛生长的组织中表达量高，在植株旺长期的成年叶中表达量也很高。