dhfr meaning in English
二氢叶酸还原酶
Examples
- An about 550 bps and an about 700 bps fragment could be seen after the vector pqe80l / dhfr / hpd - 3 was digested by bamh i + kpn i and by bamh i + hindiii r
经w亡sternblot证实，在大小分别约为3ikd和26kd的位置有两条清楚的蛋白带。 - The recombinant vector pqe - 80l / dhfr / hpd - 3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy . 3
同时还将表达运载蛋白dhfr的基因单独连接于表达载体pqe - 80l ，得到对照质粒pqe - 80l dhfr 。 - ( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr . an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii
Sds一队ge分析可见重组原核表达载体pqe一80l / dhf侧hpd一3表达的目的蛋白带和对照质粒pqe一sol / dhfr表达的蛋白带，大小分别约为31kd和26kd 。 - 2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
将自puc18 h d - 3回收的目的片段连同表达运载蛋白dhfr的基因片断一起连接于原核高效表达载体pqe - 80l ，经酶切鉴定，得到重组原核表达载体pqe - 80l dhfr h d - 3 。