creatinase meaning in English
肌酶
肌酸酶
肌酸脱水酶
Examples
- The paper deals with the isolation and identification of a creatinase - producing bacterium from soil , the conditions for production of creatinase , purification methods of creatinase and the purified enzyme characteristics
1 、产肌酸水解酶微生物的分离及鉴定本研究用恒化培养的方法从土壤中富集微生物，然后再用平板划线分离。 - Then enzyme was purified with a deae - cellulose ( 5 . 5x50cm ) column , a toyopearl hw - 65 ( 5 . 5 x 50cm ) column and a sephadex g - 200 ( 5 . 5 x 80cm ) column . finally , the enzyme was purified for 10 folds with the recovery of 17 . 4 % . page showed a single band for the purified creatinase
3 、肌酸水解酶的提纯酶在硫酸铵饱和度为40 80之间完全沉淀，先后经过deae - cellulose离子层析柱、 toyopearlhw - 65疏水层析柱、 sephadexg - 200分子筛层析柱层析，最终使酶提纯10倍，最终得率为17 . 4 。 - It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0 . 75 % . creatine , sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer , but creatinine and urea could not induce the creatinase production . 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源为玉米浆和蛋白胨，最佳比例为2 ： 3 ，最佳浓度为1 . 6 ；加入其它碳源时有助于菌株稳定产酶； 100ml摇瓶的最佳装液量为15ml ；肌酸、肌氨酸和氯化胆碱都能诱导菌株产酶，其中肌酸诱导产酶的效果最好，而肌酐和尿素不能诱导菌株产酶；诱导物肌酸的最适加入时间为接种培养12小时后，最适加入量为0 . 75 。