cosmids meaning in English
黏性质粒
Examples
- Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段为探针钓出了位于该区域的文库克隆，并利用指纹印迹和杂交技术将这些文库克隆排列起来，进而以位于该区域的不同位置的片段做臂构建了用于转化和接合转移用的基因置换质粒，并试图通过基因置换将该区域置换下来，但尚未得到最终结果。 - The work on physical mapping of the chromosome of s . nanchangensis ns3226 was initiated . nearly a full set of chromosomal asei - bamhi fragments of s . nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library . thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion , suggesting distinct identifications
此外，还开展了南昌链霉菌ns3226染色体物理图谱构建的前期研究工作：基本克隆到了南昌链霉菌ns3226染色体上全套的ase - bamh片段，以它们为探针从南昌链霉菌ns3226的基因文库中钓到164个阳性克隆，并从中筛选到34个ase linkingcosmids ，用bamh进行初步的酶谱分析，结果表明其中有20个cosmids的bamh酶谱相互间没有明显的重叠性。 - 16 replacement plasmids for two different regions were obtained , but have n ' t been introduced into 10 - 22 successfully . 42 cosmids in this region were introduced into a heterogeneous host zx64 , one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton
将300kb区域的柯斯质粒进行异源表达，发现有一文库质粒5h4的转化子在硫链丝菌素存在时表现出对棉花枯萎病菌微弱的抑制活性；无硫链丝菌素存在时，则检测不到。