bartha meaning in English
巴尔塔
鲍尔陶
Examples
- Compared to its parental virus bartha - k61 strain , it displayed no obvious differences in virus multiplication and cytopathogenic effects in different cell cultures ( pk - 15 , ibrs2 , vero and cef )
在不同的细胞上( pk - 15 、 ibrs2 、 vero和cef )对重组病毒的病毒滴度和病变与亲本毒进行比较，未发现显著差异。 - Finally , a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv . the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes . based on the expression of lacz gene , recombinant prv were selected and purified by blue - colored virus plaque
利用脂质体介导的方法，将pltk - ha与prvbartha - k61基因组共转染于亚单层vero细胞，依据报告基因lacz在细胞中的表达，筛选蓝色蚀斑的重组病毒，经数次蚀斑纯化后， pcr鉴定、 western - blot分析。 - In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株，在通用prv转移载体pbdtk - uni的基础上，在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒，同时将下游同源臂增加了一个1 . 7kb的kpni片段，使上下游同源臂的长度都超过了1kb ，构建了一个新的转移载体puni - lacz 。 - After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞，采用甲基纤维素固定病变， x - gal染色，经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株，命名为rprv - lacz 。