aved meaning in English
奥韦德
Examples
- In order to obtain a dna fragment containing aved gene , 5 ' end primer and 3 ' end primer , consisting of 18 and 20 nucleotides respectively , were designed and synthesized . a 2 . 2 kb dna fragment containing aved gene was obtained by pcr when genomic dna of streptomyces avermitilis s - 2 was used as a template
为了得到aved基因片段，首先根据已知的阿维菌素生物合成基因簇的核苷酸序列，设计并合成了由18和20个核苷酸组成的5端和3端引物；以streptomycesavermitiliss - 2基因组dna为模板，经pcr扩增得到了2 . 2kb的含有aved基因的dna片段。 - A 1 . 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e . coli / streptomyces shuttle vector with conjugation function ( containing orit gene ) . as a result of above procedures , a recombinant plasmid pid03 was obtained
将1 . 5kb的安普霉素抗性基因片段插入到aved基因中的nrui酶切位点，再将此灭活的aved基因片段插入到具有接合转移功能(含有orit基因)的链霉菌?大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pid03 。 - In this study , the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering . insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins . when avec gene was inactivated by the same way , four " 1 " components were lost and only " 2 " components , the potential precursor of ivermectin , were accumulated
将该基因簇中的aved基因通过插入外源的安普霉素抗性基因片段使其失活，导致发酵产物中4个a组分(不需要的组分)的消失；将基因簇中的avec基因通过同样手段，使其失活，导致发酵产物中4个“ 1 ”组分的消失，而主要积累“ 2 ”组分(进一步改造可成为伊维菌素的前体b _ 2组分) 。