arod meaning in English
阿罗德
Examples
- Arod airborne radar orbital determination
机载雷达轨道测定 - 4 . engineering dhqase ( arod ) - deficient e . coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e . coli chromosome . the mutant 31bk was engineered , in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e . coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system . the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene , so it improved carbon flow into the quinic acid biosynthesis direction
构建宿主菌基因精确定位突变株31bk ( arod : : arobkan ~ r )为了改变代谢途径脱氢奎尼酸( dhq )分支点上的代谢流量，使之充分流向目的产物奎尼酸合成方向，利用基因打靶技术构建了31884宿主菌arod基因精确定位插入突变体，使dhq脱水酶( dhqase )失活，阻断了碳代谢流流向芳香氨基酸生成的方向，同时用同源重组的方法将arob基因定位整合入染色体上，解除了限速酶对碳代谢流通过共同途径到达dhq的阻遏影响，并减轻代谢负担。 - Quinic acid , used shikimate pathway in e . coli , it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell . double specific enzyme genes arog , qutb or three ones arog , qutb , arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions . modifications of e . coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch
利用大肠杆菌莽草酸途径合成新的代谢物奎尼酸，须在宿主细胞引入异源酶基因扩展代谢途径；串联表达酶基因，同时适量增加不同种属的多个关键酶酶量，改善限速反应；利用同源重组进行基因整合和基因破坏，改造染色体结构定向改变微生物代谢途径；目的是将碳代谢流最大程度的引向奎尼酸生成的方向。