anti-hbsag meaning in English
antigen]抗乙肝表面抗原的抗体
抗乙肝表面抗原的抗体
Examples
- Conclusions : ( 1 ) five human anti - hbsag scfv genes have been successfully constructed
结论门）成功地构建了五种人抗hbsag单链抗体基因。 - Objective : to construct the single - chain fv gene of human anti - hbsag and to analyse the expression of the constructed gene in eukaryotic cell and prokaryotic cell
目的构建人抗hbsag单链抗体基因，在真核细胞和原核细胞中进行表达，并对其表达产物的活性进行鉴定。 - It is also applied to determine hepatitis b surface antigen using the sensor chip inunobilized with anti - hbsag monoclonal anibody . to compare with spr method , the same hbsag solotions are determined using the method of enzyme - linked immuosorbent assay ( ellsa ) . the kinatics of inununo - reaction is studied using the spr sensol in the second task , the theory of the sensing technology is investigated
实验结果表明： spr传感器具有良灯的重现性，其灵敏度为1844iun riu ，对折射率的分辨能力为5xio ” aiu ； x利用该spr传感器测定了两种葡萄糖注射液，将检测结果与药典法中所采用的标准方法进行了对比，实验结果表明了两种方法的相对误差分别为1刀5和1 - Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。