amv meaning in English
鸟类成髓细胞白血病簿
禽白血病毒组
禽成髓细胞增多症病毒
苜蓿花叶簿
Examples
- Comparing with using mulv reverse transcriptase or amv reverse transcriptase to do reverse transcription at 3 7 - 42 " c , more accurate and complete dna could be gotten by using tag dna polymerase at 72 c since dimeric structure of mrna was opened fully at high temperature
与在37 - 42用mulv或amv进行反转录相比,在较高温度下( 72 )用taqdna聚合酶进行反转录有利于正确、完整的cdna ,因为在高温下mrna的二级结构被充分打开。 - Firstly , the major ampullate glands were got from araneus ventricousus . total rna and mrna of ampullate glands were isolated and purified . double strands cdnas were synthesized in the assistance of amv - rt and e . coli dna polymerase i etc . by reverse transcription and replacement synthesis
首先剥离大腹园蛛主壶腹腺,提取总rna和分离纯化mrna ,反转录合成cdna ,凝胶层析除去小于400bp片段,并在双链cdna两端引入ecor的衔接头,对其进行磷酸化处理。