ampl meaning in English
放大器
Examples
- Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control
选取单个菌落分别点种到删平板和md平板,找出在回d上生长正常, w上生长缓慢或不生长的菌落,即阳性菌落,再以阳性菌落分别涂布zeocine含量500ug加, 1000ug砌l的ypd平板,以高浓度的抗生素筛选高拷贝的酵母工程菌,在含500ug ml高浓度抗生素平板上获得了15个转化子,取其中5个进行pcr扩增,有3个扩增产物与阳性对照相同,说明此酵母细胞中已含有s hbsag融合片段,其中之一命名为p