aminosilane meaning in English
氨基硅烷
Examples
- Aminosilane coupling agent
氨基硅烷偶合剂 - The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide , and some factors affect immobile ratio of oligonucleotide probe , such as aminosilane treatment time , aldehyde treatment time , uv crosslinking energy , washing temperature and time
研究表明，氨基化试剂浓度对玻片荧光背景有影响，氨基化试剂处理时间、醛基化处理时间、紫外交联能量和洗涤温度和时间等工艺因素影响寡核苷酸探针的固定率。 - The key stage of fabricating gene chip is pretreatment of glass surface including the processes of nh3h2o treatment , aminosilane treatment and aldehyde treatment . the pretreatment can grow active group that can bind probe effectively on the surface of glass slide . as a result , the actively treated glass slide can suit for fabricating in - situ synthesis high density gene chips
基因芯片制备技术的关键步骤是玻片表面预处理，即对玻片表面进行羟基化、氨基化和醛基化处理，使表面生长的活性基团能有效固定寡核苷酸探针，以满足原位合成高密度基因芯片对玻片的要求。 - The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide . the more oligonucleotide probes have been linked with active group , the stronger fluorescence intensity is . for the strongest fluorescence , the technical conditions is : treatment of 2 % aminosilane of 20 minutes , treatment of 5 % aldehyde of 24 minutes , uv crosslinking of 150mj and washing of 5 minutes at 20
两种检测方法表明，当活性基团呈柱状、分布均匀且尺寸比较大( 200nm )时，有利于寡核苷酸探针的连接，且连接探针数量多，玻片表面荧光强度强，固定率高；当活性基团呈锥状、分布及尺寸不均匀( 150nm ( 300nm )时，连接的寡核苷酸探针数量少，玻片表面荧光强度弱，固定率低。 - First , glass slides having been rinsed will be treated with nh3h2o , aminosilane and aldehyde . second , the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope . in the end , the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods
实验选用表面平整的德国玻片，将清洗好的玻片分别进行羟基化、氨基化、醛基化，采用荧光法和原子力显微镜法分别检测玻片表面预处理质量，研究两种检测方法之间的内在联系，从而确定表征玻片表面寡核苷酸探针固定率的方法。