acetosyringone meaning in English
乙酰丁香酮
Examples
- Acetosyringone ( as ) can increase the gus gene transient expression when it was added in the suspending medium
在菌液中添加as可以提高gus基因的瞬时表达。 - ( 8 ) the add of acetosyringone had little influence on transformation result . finally , we gained the transgenic plants
通过优化的转化体系，将vp7和ctb - vp7基因导入胡萝卜中，获得了阳性株。 - The best concentration of acetosyringone was about 30 m . silver nitrate below 4 mg / l caused little change in efficiency and higher concentration ( above 10 mg / l ) strongly inhibited hairy root induction
M时，转化率不能显著提高。 4m幼以下的硝酸银便能有效地提高遗传转化率，但高剂量( 10m幼)时作用却相反。 - Acetosyringone ( as ) could dramatically improve transformation frequency , its suitable concentration is 20 mg . l " 1 . before inoculated with agrobacterium , calli should be precultured 1 ? days , and the appropriate inoculation time is 4 ~ 8 minutes , and then cocultured for 3 ~ 4days . then the cocultured calli were transferred to ms medium containing 400 mg . l " 1 cef
7为宜；侵染前预培养1 2天，可显著提高转化效率；侵染时间以4 8分钟为宜；共培养时间3 4天较适合；转化后暗培养对转化效率没有影响；选择方法以延迟选择15天选择转化率最高。 - Establishment of carrot genetic transformation an efficient transformation protocol based on agrobacterium tumefacien lba4404 was created in the study . good results were gained when use fresh hypocotyls as infectious explants , co - cultivate in medium supplemented with low concentration of acetosyringone ( 25 m ) , screen in medium with loomg / l kanamycin . it is time saving when anti - culli are fisrt screened in low concentration of antibiotics then transfered to high concentration ones , and remove antibiotics when regenerate
建立了高效的遗传转化体系以pbi121和ptok233为转化质粒，在农杆菌lba4404介导下，对胡萝卜遗传转化体系进行系统研究，首次为该胡萝卜品种建立一套高效的遗传转化体系，结果为:最适转化受体为新鲜下胚轴及经预培养的下胚轴;共培养时，低浓度的乙酞丁香酮( 25pm )对转化具有促进作用;适宜的卡那霉素筛选浓度是loom岁l 。