acci meaning in English

阿奇

And then a 378bp acci fragment in pbtk2 . 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni , a eukaryotic expression plasmid
在此基础上，进一步用tthlll酶切后，补平插入带有sv40启动子的大肠杆菌?半乳糖苷酶报一告基因( lacz ) ，获得通用转移载体pltk - uni 。
In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株，在通用prv转移载体pbdtk - uni的基础上，在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒，同时将下游同源臂增加了一个1 . 7kb的kpni片段，使上下游同源臂的长度都超过了1kb ，构建了一个新的转移载体puni - lacz 。